ENO2 Antibody
ENO2 Antibody
Product Specifications
Product Name Alternative
ENO2, Enolase 2, Enolase 2 (gamma, neuronal), Neurone-specific enolase, Gamma-enolase, Neural enolase, Neuron specific gamma enolase, Neuron-specific enolase, NSE
UniProt
P09104
Reactivity
Human
Immunogen
Amino acids 416-433 of human Neuron-specific Enolase were used as the immunogen for this NSE antibody.
Target
ENO2
Clonality
Monoclonal
Clone
NSEL1-1
Conjugation
Unconjugated
Field of Research
Metabolism Research
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Western blot: 1-2 μg/mLFlow Cytometry: 0.5-1 ug/million cellsIF: 1-2 μg/mLIHC (FFPE) : 0.5-1 μg/mL (1) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NSE antibody to be titered up or down for optimal performance.1. Staining of FFPE tissue requires boiling sections in 10mM Citrate Buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
Tested Applications
FC, IF, IHC, WB
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG2b, kappa
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