TOP2A Antibody

Product Specifications

Product Name Alternative

DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme, TOP2A, TOP2

UniProt

P11388

Reactivity

Human

Immunogen

Human partial recombinant protein was used as the immunogen for this TOP2A antibody.

Target

TOP2A

Clonality

Monoclonal

Clone

TPM2A-1

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Flow Cytometry: 0.5-1ug/10^6 cellsIF: 1-2 μg/mLWestern blot: 1-2 μg/mLIHC (FFPE) : 0.5-1 μg/mL (1) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TOP2A antibody to be titered up or down for optimal performance.1. Staining of FFPE tissue requires boiling sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.