AFP Antibody
AFP Antibody
Product Specifications
Product Name Alternative
AFP Antibody: FETA, HPAFP, Alpha-fetoprotein, Alpha-1-fetoprotein
UniProt
P02771
Reactivity
Canine, Human, Monkey, Porcine
Immunogen
Alpha fetoprotein purified from serum of a hepatoma patient was used as the immunogen for this AFP antibody.
Target
AFP
Clonality
Monoclonal
Clone
SPM334
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 μg/mLImmunohistochemistry (FFPE) : 1-2 μg/mL for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) The optimal dilution of the Alpha Fetoprotein antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FC, IF, IHC
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG2a, kappa
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