ECM1 Antibody
ECM1 Antibody
Product Specifications
UniProt
Q16610
Reactivity
Human, Rat
Immunogen
Secretory Component protein isolated from human colostrum was used as the immunogen for this Secretory Component antibody.
Target
ECM1
Clonality
Monoclonal
Clone
SPM217
Conjugation
Unconjugated
Field of Research
Cardiovascular Research
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 μg/mLImmunohistochemistry (FFPE) : 0.5-1 μg/mL for 30 minutes at RT (1) Prediluted format: incubate for 30 min at RT (2) The optimal dilution of the Secretory Component antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FC, IF, IHC
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG1, kappa
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