INS Antibody

Product Specifications

Product Name Alternative

INS, IRDN, IDDM2, Preproinsulin, ILPR, Insulin, MODY10, Proinsulin

UniProt

P01308

Reactivity

Bovine, Human, Porcine, Rabbit, Rat

Immunogen

Full length (amino acids 1-84) purified pig insulin was used as the immunogen for this anti-Insulin antibody.

Target

INS

Clonality

Monoclonal

Clone

SPM531

Conjugation

Unconjugated

Field of Research

Metabolism Research

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 μg/mLImmunohistochemistry (FFPE) : 0.5-1 μg/mL for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) The optimal dilution of the anti-Insulin antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.