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KRT10 Antibody

KRT10 Antibody

Product Specifications

Product Name Alternative

Keratin, type I cytoskeletal 10, Cytokeratin-10, CK-10, Keratin-10, K10, KRT10, KPP

UniProt

P13645

Reactivity

Human, Mouse

Immunogen

Skin extract of a human Psoriasis patient were used as the immunogen for this Cytokeratin 10 antibody.

Target

KRT10

Clonality

Monoclonal

Clone

SPM261

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 μg/mLImmunohistochemistry (FFPE) : 0.1-0.2 μg/mL for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) The optimal dilution of the Cytokeratin 10 antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

FC, IF, IHC

Host or Source

Mouse

Preservative

PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

IgG1, kappa

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