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KRT14 Antibody

KRT14 Antibody

Product Specifications

Product Name Alternative

Keratin, type I cytoskeletal 14, Cytokeratin-14, CK-14, Keratin-14, K14, KRT14

UniProt

P02533

Reactivity

Human, Mouse, Rat

Immunogen

A 15 amino acid peptide from the C-terminus of human keratin 14 was used as the immunogen for this anti-Cytokeratin 14 antibody.

Target

KRT14

Clonality

Monoclonal

Clone

SPM263

Conjugation

Unconjugated

Field of Research

Cancer Biology, Cell Biology, Signal Transduction

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 μg/mLImmunohistochemistry (FFPE) : 0.5-1 μg/mL for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) The optimal dilution of the anti-Cytokeratin 14 antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

FC, IF, IHC

Host or Source

Mouse

Preservative

PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

IgG3

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