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VWF Antibody

VWF Antibody

Product Specifications

Product Name Alternative

VWF, Coagulation factor VIII VWF, F8VWF, VWD, Von Willebrand factor

UniProt

P04275

Reactivity

Human

Immunogen

A partial recombinant human protein was used as the immunogen for this anti-von Willebrand Factor antibody.

Target

VWF

Clonality

Monoclonal

Clone

SPM577

Conjugation

Unconjugated

Field of Research

Cardiovascular Research

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 μg/mLWestern blot: 0.5-1 μg/mLImmunoprecipitation: 0.5-1 ug/500ug protein lysateImmunohistochemistry (FFPE) : 0.5-1 μg/mL for 30 minutes at RT (1) The optimal dilution of the anti-von Willebrand Factor antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Tested Applications

FC, IF, IHC, IP, WB

Host or Source

Mouse

Preservative

PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

IgG1, kappa

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