Golgi Antibody [SPM581]
Golgi Antibody [SPM581]
Product Specifications
Reactivity
Human
Immunogen
SU-DHL-1 large cell lymphoma cells were used as the immunogen for this anti-Golgi antibody.
Clonality
Monoclonal
Clone
SPM581
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Flow Cytometry (0.5-1 ug/million cells in 0.1ml) Immunofluorescence: 0.5-1 μg/mLWestern blot: 0.5-1 μg/mLICC (Acetone or paraformaldehyde fixed) : 0.5-1 μg/mL for 30 minImmunohistochemistry (FFPE) : 0.5-1 μg/mL for 30 minutes at RT (1) The optimal dilution of the anti-Golgi antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FC, ICC, IF, IHC, WB
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG1, kappa
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