Multi-Cytokeratin Antibody [SPM583]
Multi-Cytokeratin Antibody [SPM583]
Product Specifications
UniProt
P48668, P19013, P13645, P05783, P04259, P05787, P02538, P13647, P13646
Reactivity
Bovine, Frog, Goat, Guinea pig, Human, Marmoset, Mouse, Porcine, Rat
Immunogen
A keratin-enriched preparation from cultured human A431 cells was used as the immunogen for this anti-Multi-Cytokeratin antibody.
Target
KRT4, KRT5, KRT6A, KRT6B, KRT8, KRT10, KRT13, KRT18
Clonality
Monoclonal
Clone
SPM583
Conjugation
Unconjugated
Field of Research
Signal Transduction
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 μg/mLWestern blot: 0.5-1 μg/mLImmunohistochemistry (FFPE) : 0.5-1 μg/mL for 30 min at RT (1) Prediluted format: incubate for 30 min at RT (2) The optimal dilution of the anti-Multi-Cytokeratin antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FC, IF, IHC, WB
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG1
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