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Biotin Antibody [SPM375]

Biotin Antibody [SPM375]

Product Specifications

Reactivity

All

Immunogen

Biotinylated sheep immunoglobulin was used as the immunogen for this anti-Biotin antibody.

Clonality

Monoclonal

Clone

SPM375

Conjugation

Unconjugated

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Confocal MicroscopyFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 μg/mLIn situ Nucleic Acid HybridizationWestern: blot 1-3 μg/mLImmunohistochemistry (FFPE) : 0.5-1 μg/mL for 30 minutes at RT (1) The optimal dilution of the anti-Biotin antibody for each application should be determined by the researcher.1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.

Tested Applications

CM, FA, FC, IF, IHC, WB

Host or Source

Mouse

Preservative

PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

IgG1

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