Biotin Antibody [SPM375]
Biotin Antibody [SPM375]
Product Specifications
Reactivity
All
Immunogen
Biotinylated sheep immunoglobulin was used as the immunogen for this anti-Biotin antibody.
Clonality
Monoclonal
Clone
SPM375
Conjugation
Unconjugated
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Confocal MicroscopyFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 μg/mLIn situ Nucleic Acid HybridizationWestern: blot 1-3 μg/mLImmunohistochemistry (FFPE) : 0.5-1 μg/mL for 30 minutes at RT (1) The optimal dilution of the anti-Biotin antibody for each application should be determined by the researcher.1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Tested Applications
CM, FA, FC, IF, IHC, WB
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG1
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