Bromodeoxyuridine Antibody [SPM166]

Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 μg/mLImmunohistochemistry (FFPE) : 0.5-1 μg/mL for 30 minutes at RT (1) Prediluted format: incubate for 30 min at RT (2) Prediluted format: incubate for 30 min at RT (2) The optimal dilution of the anti-Bromodeoxyuridine antibody for each application should be determined by the researcher.1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with Trypsin at 1mg/ml PBS, 10 min at 37oC.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.