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Hepatocyte Antibody [SPM582]

Hepatocyte Antibody [SPM582]

Product Specifications

Reactivity

Canine, Human

Immunogen

Extract of a formalin-fixed rejected-allograft of human liver was used a the immunogen for this Hepatocyte antibody.

Clonality

Monoclonal

Clone

SPM582

Conjugation

Unconjugated

Field of Research

Gastroenterology & Hepatology

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Immunofluorescence (0.5-1 μg/mL) Immunohistochemistry (FFPE) : 1-2 μg/mL for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) The optimal dilution of the Hepatocyte antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IF, IHC

Host or Source

Mouse

Preservative

PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

IgG1

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