Hepatocyte Antibody [SPM582]
Hepatocyte Antibody [SPM582]
Product Specifications
Reactivity
Canine, Human
Immunogen
Extract of a formalin-fixed rejected-allograft of human liver was used a the immunogen for this Hepatocyte antibody.
Clonality
Monoclonal
Clone
SPM582
Conjugation
Unconjugated
Field of Research
Gastroenterology & Hepatology
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Immunofluorescence (0.5-1 μg/mL) Immunohistochemistry (FFPE) : 1-2 μg/mL for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) The optimal dilution of the Hepatocyte antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
IF, IHC
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG1
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