CheKine™ Mitochondrial Reactive Oxygen Species (ROS) Production Rate Fluorometric Assay Kit

Reactive oxygen species (ROS) are natural by-products of normal oxygen metabolism, including superoxide radicals, hydrogen peroxide, and its downstream products peroxides and hydroxides. Studies have shown that more than 95% of ROS in the body come from mitochondria, and the oxidative stress caused by its imbalance is related to cell growth and proliferation, developmental differentiation, aging and apoptosis, as well as many physiological and pathological processes. Under normal conditions, the intracellular antioxidant defense system is in balance with oxygen free radicals, and the level of intracellular ROS is maintained in a low physiological range. In pathological conditions, the balance between the intracellular antioxidant system and oxygen free radicals is disrupted. Excessive levels of ROS in cells can disrupt mitochondrial enzymes, lipids, and nucleic acids, leading to oxidative stress in the body. Meanwhile, ROS can also attack mitochondrial DNA and cause oxidative damage, resulting in structural and functional changes such as reduced mitochondrial ATP synthesis and mitochondrial membrane potential destruction. CheKine™ provides a simple, sensitive, and rapid method for detecting the rate of mitochondrial ROS production. The principle is to use fluorescent probe DCFH-DA for reactive oxygen species detection. DCFH-DA (2’,7’-Dichlorofluorescin Diacetate) can diffuse through the mitochondrial membrane and be hydrolyzed by esterase in mitochondria to form non fluorescent DCFH. DCFH is oxidized by ROS to produce fluorescent DCF, and the rate of increase in fluorescence intensity of DCF is proportional to the rate of ROS production.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Storage at -20°C and Keep from light immediately upon receipt. Kit has a storage time of 12 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

• If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples.