Anti-ENaC α Antibody

Mouse Monoclonal Antibody specific to ENaC α

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-ENaC α Mouse Monoclonal Antibody

Gene Name

Scnn1a

NCBI Gene ID

UniProt

Q6IRJ1

Accession Number

NP_113736

Host

Mouse

Reactivity

Mouse, Rat

Immunogen

Synthetic peptide corresponding to N-terminal aa 46-68 of rat ENaC alpha.

Target Antigen

Amiloride sensitive sodium channel α1 subunit

Target

ENaC α

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC

Field of Research

Ion Channels

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: use at 1ug/mL. Immunofluorescence:Detection of ~85kDa ENaC alpha with #11574 at 1ug/mL.Detection of ENaC alpha (green) in paraffin- embedded, formalin-fixed rat kidney with #11574 at 10ug/mL. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

Mouse and rat ENaC alpha

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Concentration: 1mg/ml

Buffer pH

pH 7.4

Target Background

The epithelial sodium channel (ENaC) is a membrane-bound ion-channel that is selectively permeable to Na+ ions and that is assembled as a heterotrimer composed of three homologous subunits alpha, beta, and g. It is involved primarily in the reabsorption of sodium ions in the collecting ducts of the kidney's nephrons. These channels mediate the first step of active sodium reabsorption essential for the maintenance of body salt and water homeostasis. In vertebrates, the channels control reabsorption of sodium in kidney, colon, lung and sweat glands; they also play a role in taste perception.