DOTMA

DOTMA is a cationic lipid that has been used as a non-viral vector for gene therapy. DOTMA is used as a component of liposomes to encapsulate siRNA, microRNA, and oligonucleotides and for in vitro gene transfection. DOTMA promotes effective interaction between liposomes and cell membranes by inducing positive charge on the liposomes. DOTMA showed good gene transfection effect both in vitro and in vivo[1][2][3][4][5].

Product Specifications

CAS Number

[104872-42-6]

UNSPSC

12352211

Hazard Statement

H315, H319

Target

Liposome

Type

Oligonucleotides

Related Pathways

Metabolic Enzyme/Protease

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/dotma.html

Purity

98.0

Solubility

DMSO : 12.5 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

CCCCCCCC/C=C/CCCCCCCCOCC(C[N+](C)(C)C)OCCCCCCCC/C=C/CCCCCCCC.[Cl-]

Molecular Formula

C42H84ClNO2

Molecular Weight

670.57

Precautions

H315, H319

References & Citations

[1]Aime S, et al. Properties, solution state behavior, and crystal structures of chelates of DOTMA. Inorg Chem. 2011;50 (17) :7955-7965.|[2]Bhavsar D, et al. Translational siRNA therapeutics using liposomal carriers: prospects & challenges. Curr Gene Ther. 2012;12 (4) :315-332.|[3]Ren T, et al. Structural basis of DOTMA for its high intravenous transfection activity in mouse. Gene Ther. 2000;7 (9) :764-768.|[4]Wang X, et al. Enhanced hepatic delivery of siRNA and microRNA using oleic acid based lipid nanoparticle formulations. J Control Release. 2013 Dec 28;172 (3) :690-8.|[5]Huang QD, et al. Cyclen-based cationic lipids for highly efficient gene delivery towards tumor cells. PLoS One. 2011;6 (8) :e23134.