TIMP3 Antibody
TIMP3 Antibody
Product Specifications
Product Name Alternative
Metalloproteinase inhibitor 3, Protein MIG-5, Tissue inhibitor of metalloproteinases 3, TIMP-3, TIMP3
UniProt
P35625
Reactivity
Bovine, Canine, Equine, Human, Mouse, Rat
Immunogen
Amino acids 175-211 of human TIMP3 Ab was used as the immunogen for this antibody (100% homologous in cow, dog and horse, and 94% homologous in mouse and rat) .
Target
TIMP3
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology, Cell Biology, Neuroscience, Signal Transduction
Purification
Protein A affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Flow Cytometry: use antibody at 0.5-1 ug/million cellsIF: 1-2 μg/mLWB: 0.5-1 μg/mLIP: 1-2ug/500ug protein lysateImmunohistochemistry (FFPE) : 1-2 μg/mL for 30 minutes at RT (1) Prediluted format: incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TIMP3 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FC, IF, IHC, IP, WB
Host or Source
Rabbit
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG
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