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TAG-72 Antibody [B72.3]

TAG-72 Antibody [B72.3]

Product Specifications

Reactivity

Bovine, Canine, Hamster, Human, Rat

Immunogen

The membrane-enriched fraction of a human breast carcinoma liver metastasis was used as the immunogen for this TAG-72 antibody.

Clonality

Monoclonal

Clone

B72.3

Conjugation

Unconjugated

Field of Research

Cancer Biology, Signal Transduction

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 μg/mLIHC (FFPE) : 0.5-1 μg/mL for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TAG-72 antibody to be titered up or down for optimal performance.1. No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

FC, IF, IHC

Host or Source

Mouse

Preservative

PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

IgG1, kappa

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