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AFP Antibody

AFP Antibody

Product Specifications

Product Name Alternative

AFP Antibody: FETA, HPAFP, Alpha-fetoprotein, Alpha-1-fetoprotein

UniProt

P02771

Reactivity

Human

Immunogen

Recombinant full-length human Alpha Fetoprotein was used as the immunogen for the AFP antibody.

Target

AFP

Clonality

Monoclonal

Clone

MBS-12

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 μg/mLImmunohistology (FFPE) : 1-2 μg/mL for 30 minutes at RT (1) Prediluted format : incubate for 30 min at RT (2) Optimal dilution of the AFP antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

FC, IF, IHC-P, WB

Host or Source

Mouse

Preservative

PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

IgG1, kappa

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