IgG Antibody [B33/20]
IgG Antibody [B33/20]
Product Specifications
UniProt
P01860, P01861, P01859, P01857
Reactivity
Human
Immunogen
Purified polyclonal human Ig Gamma Chain was used as the immunogen for the Anti-IgG antibody.
Clonality
Monoclonal
Clone
B33/20
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 μg/mLImmunohistochemistry (Formalin/paraffin) : 0.5-1 μg/mL for 30 min at RT (1) Prediluted format : incubate for 30 min at RT (2) Optimal dilution of the Anti-IgG antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Tested Applications
FC, IF, IHC-P
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG1, kappa
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