Double Stranded DNA Antibody [SPM603]

Product Specifications

Reactivity

Human

Immunogen

Nuclei of Burkitt's cells were used as the immunogen for the Double Stranded DNA antibody.

Clonality

Monoclonal

Clone

SPM603

Conjugation

Unconjugated

Purification

Protein G affinity chromatography

Concentration

0.2 mg/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 μg/mLImmunocytochemistry (Acetone-fixed) : 0.5-1 μg/mL for 30 min at RTImmunohistochemistry (FFPE) : 1-2 μg/mL for 30 min at RT (1) Optimal dilution of the Double Stranded DNA antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.