CD28 Antibody
CD28 Antibody
Product Specifications
Product Name Alternative
T-cell-specific surface glycoprotein CD28, TP44, CD28, CD28
UniProt
P10747
Reactivity
Human
Immunogen
Recombinant human CD28 protein was used as the immunogen for this antibody.
Target
CD28
Clonality
Monoclonal
Clone
C28/77
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Protein G affinity chromatography
Concentration
0.2 mg/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 μg/mLIHC (FFPE) : 0.5-1 μg/mL for 30 minutes at RT (1) The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 8.5-9.5, for 10-20 min followed by cooling at RT for 20 minutes.
Tested Applications
FC, IF, IHC, WB
Host or Source
Mouse
Preservative
PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Isotype
IgG1, kappa
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