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POLQ Antibody

POLQ Antibody

Product Specifications

Product Name Alternative

POLQ , polymerase (DNA directed), theta , DKFZp781A0112, POLH, PRO0327, DNA polymerase eta, DNA polymerase theta

UniProt

O75417

Immunogen

The immunogen for this antibody is: DTHQQLTCSNDES

Target

POLQ

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Epigenetics & Chromatin

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Preliminary experiments in Human Brain (Cerebellum lysates) and in lysates of cell line HeLa gave no specific signal but low background (at antibody concentration up to 1μg/mL) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Prediction Reactivity

Human

Tested Applications

ELISA

NCBI Accession Number

NP_955452.3

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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