POLQ Antibody
POLQ Antibody
Product Specifications
Product Name Alternative
POLQ , polymerase (DNA directed), theta , DKFZp781A0112, POLH, PRO0327, DNA polymerase eta, DNA polymerase theta
UniProt
O75417
Immunogen
The immunogen for this antibody is: DTHQQLTCSNDES
Target
POLQ
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Preliminary experiments in Human Brain (Cerebellum lysates) and in lysates of cell line HeLa gave no specific signal but low background (at antibody concentration up to 1μg/mL) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Prediction Reactivity
Human
Tested Applications
ELISA
NCBI Accession Number
NP_955452.3
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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