Pyronin Y

Pyronin Y (Pyronine G) is a cationic dye that intercalates RNA and has been used to target cell structures including RNA, DNA and organelles. Pyronin Y forms fluorescent complexes with double-stranded nucleic acids (especially RNA) enabling semi-quantitative analysis of cellular RNA. Pyronin Y can be used to identify specific RNA subspecies of ribonuclear proteins complexes in live cells[1][2][5].

Product Specifications

CAS Number

[92-32-0]

Product Name Alternative

Pyronine G; C.I. 45005

UNSPSC

12171500

Hazard Statement

H301, H315, H319, H341

Target

DNA Stain

Type

Dye Reagents

Related Pathways

Cell Cycle/DNA Damage

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/Pyronin_Y.html

Purity

82.2

Solubility

DMSO : 25 mg/mL (ultrasonic) |H2O : 4 mg/mL (ultrasonic)

Smiles

CN(C1=CC2=[O+]C3=C(C=CC(N(C)C)=C3)C=C2C=C1)C.[Cl-]

Molecular Formula

C17H19ClN2O

Molecular Weight

302.80

Precautions

H301, H315, H319, H341

References & Citations

[1]Pollack A, et al. Flow cytometric analysis of RNA content in different cell populations using pyronin Yand methyl green. Cytometry. 1982 Jul;3 (1) :28-35.|[2]Tanke HJ, et al. Flow cytometry of human reticulocytes based on RNA fluorescence. Cytometry. 1981 Mar;1 (5) :313-20.|[3]Darzynkiewicz Z, et al. Cytostatic and cytotoxic properties of pyronin Y: relation to mitochondrial localization of the dye and its interaction with RNA. Cancer Res. 1986 Nov;46 (11) :5760-6.|[4]Li B, et al. Pyronin Y as a fluorescent stain for paraffin sections. Histochem J. 2002 Jun-Jul;34 (6-7) :299-303.|[5]Laura M Andrews, et al. Spectral phasor analysis of Pyronin Y labeled RNA microenvironments in living cells. Biomed Opt Express. 2013 Jan 1;4 (1) :171-7.