Diphenyl Blue
Diphenyl Blue (Trypan Blue) is a cell active dye, the most commonly used dye for the identification of dead cells, of en used to test cell membrane integrity and cell viability. Diphenyl Blue staining is one of the methods for tissue and cell culture. When cells are deactivated or have incomplete cell membranes, Diphenyl Blue can stain them Blue. Normal living cells with intact cell membranes reject Diphenyl blue and do not stain them blue. However, macrophages are capable of phagocytosis of Diphenyl Blue, so it can be used as a living stain for macrophages[1].
Product Specifications
CAS Number
72-57-1
Product Name Alternative
Direct Blue 14; Trypan Blue
UNSPSC
12171500
Hazard Statement
H350
Target
Fluorescent Dye
Type
Dye Reagents
Related Pathways
Others
Field of Research
Others
Assay Protocol
https://www.medchemexpress.com/Diphenyl_Blue.html
Purity
0
Solubility
DMSO : 25 mg/mL (ultrasonic; warming; heat to 60°C) |H2O : 25 mg/mL (ultrasonic; warming; heat to 60°C)
Smiles
CC1=CC(C2=CC=C(/N=N/C3=C(S(=O)(O[Na])=O)C=C4C=C(S(=O)(O[Na])=O)C=C(N)C4=C3O)C(C)=C2)=CC=C1/N=N/C5=C(S(=O)(O[Na])=O)C=C6C=C(S(=O)(O[Na])=O)C=C(N)C6=C5O
Molecular Formula
C34H24N6Na4O14S4
Molecular Weight
960.81
Precautions
H350
References & Citations
Shipping Conditions
Room Temperature
Storage Conditions
4°C (Powder, sealed storage, away from moisture and light)
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Scientific Category
Dye Reagents
Clinical Information
No Development Reported
Available Sizes
Frequently Asked Questions
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