Diphenyl Blue

Diphenyl Blue (Trypan Blue) is a cell active dye, the most commonly used dye for the identification of dead cells, of en used to test cell membrane integrity and cell viability. Diphenyl Blue staining is one of the methods for tissue and cell culture. When cells are deactivated or have incomplete cell membranes, Diphenyl Blue can stain them Blue. Normal living cells with intact cell membranes reject Diphenyl blue and do not stain them blue. However, macrophages are capable of phagocytosis of Diphenyl Blue, so it can be used as a living stain for macrophages[1].

Product Specifications

CAS Number

[72-57-1]

Product Name Alternative

Direct Blue 14; Trypan Blue

UNSPSC

12171500

Hazard Statement

H350

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/Diphenyl_Blue.html

Purity

Solubility

DMSO : 25 mg/mL (ultrasonic; warming; heat to 60°C) |H2O : 25 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

CC1=CC(C2=CC=C(/N=N/C3=C(S(=O)(O[Na])=O)C=C4C=C(S(=O)(O[Na])=O)C=C(N)C4=C3O)C(C)=C2)=CC=C1/N=N/C5=C(S(=O)(O[Na])=O)C=C6C=C(S(=O)(O[Na])=O)C=C(N)C6=C5O

Molecular Formula

C34H24N6Na4O14S4

Molecular Weight

960.81

Precautions

H350

References & Citations

[1]Daly, M. L., DeRosa, C. A., Kerr, C., Morris, W. A., & Fraser, C. L. (2016) . Blue thermally activated delayed fluorescence from a biphenyl difluoroboron β-diketonate. RSC Advances, 6 (85), 81631–81635. doi:10.1039/c6ra18374c