IREB2 Antibody
IREB2 Antibody
Product Specifications
Product Name Alternative
IREB2, iron-responsive element binding protein 2, ACO3, FLJ23381, IRP2, IRP2AD, iron regulatory protein 2
UniProt
P48200
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-SIHYEGSEYKLSHGS
Target
IREB2
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 110kDa band observed in Human Liver lysates (calculated MW of 105kDa according to NP_004127.1) . Recommended concentration: 1-3μg/mL. An additional band of unknown identity was also consistently observed at 60kDa. This band was confirmed as a breakdown product in aged purified recombinant protein by a free sample recipient.Immunohistochemistry:In paraffin embedded Human Kidney shows preferential staining in the cytoplasm of DCT. Recommended concentration, 3-6μg/mL.
Prediction Reactivity
Mouse, Rat
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_004127.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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