ICOS Antibody
ICOS Antibody
Product Specifications
Product Name Alternative
ICOS, inducible T-cell co-stimulator, AILIM, CD278, MGC39850, activation-inducible lymphocyte immunomediatory molecule, inducible costimulator
UniProt
Q9Y6W8
Reactivity
Human
Immunogen
The immunogen for this antibody is: KGGQILCDLTKTKGS
Target
ICOS
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 13kDa band in Human Peripheral Blood Lymphocyte lysates after 0.01μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 22.6kDa according to NP_036224.1. The 13kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:In paraffin embedded Human Lymph Node shows membrane staining just outside the mantle zone of the Germinal Centre. Recommended concentration: 1-2μg/mL. Paraffin embedded Human Tonsil and Thymus. Recommended concentration: 3.75μg/mL.
Tested Applications
ELISA, IHC
NCBI Accession Number
NP_036224.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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