UBE2F Antibody
UBE2F Antibody
Product Specifications
Product Name Alternative
UBE2F, ubiquitin-conjugating enzyme E2F (putative), MGC18120, NCE2, NEDD8 conjugating enzyme, NEDD8-conjugating enzyme
UniProt
Q969M7
Reactivity
Human, Mouse
Immunogen
The immunogen for this antibody is: C-HLRDKEDFRNKVDD
Target
UBE2F
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 20kDa band observed in Mouse Liver lysates (calculated MW of 21.1kDa according to Human NP_542409.1 and to Mouse NP_080730.1) . Recommended concentration: 0.05-0.2μg/mL.Immunohistochemistry:In paraffin embedded Human Placenta shows membrane staining in trophoblasts-. Recommended concentration, 2.5-3.8μg/mL.
Prediction Reactivity
Bovine, Canine, Rat
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_542409.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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