MDFI Antibody

Product Specifications

Product Name Alternative

MDFI, MyoD family inhibitor, I-MF, I-mfa, OTTHUMP00000016375, OTTHUMP00000196414, inhibitor of MyoD family a

UniProt

Q99750

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-SKSTTSQIPLQAQED

Target

MDFI

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Stem Cell & Developmental Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx. 70kDa band in lysates of cell line HepG2 after 0.3μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 25kDa according to NP_005577.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Lung (Bronchus) . Recommended concentration: 5μg/mL.

Tested Applications

ELISA, IHC

NCBI Accession Number

NP_005577.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.