COL4A6 Antibody

Product Specifications

Product Name Alternative

COL4A6, collagen, type IV, alpha 6, MGC88184, OTTHUMP00000023834, OTTHUMP00000023835, collagen IV, alpha-6 polypeptide, collagen alpha 6 type IV, collagen of basement membrane, alpha-6, dJ889N15.4 (Collagen Alpha 6 (IV) ), type IV alpha 6 collagen

UniProt

Q14031

Immunogen

The immunogen for this antibody is: C-PSPEFETETLHNKES

Target

COL4A6

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 20kDa band in Human Heart, Kidney and Skeletal Muscle lysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 164kDa according to NP_001838.2 and NP_378667.1. The 20kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Prediction Reactivity

Human

Tested Applications

ELISA

NCBI Accession Number

NP_378667.1, NP_001838.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.