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GAL Antibody

GAL Antibody

Product Specifications

Product Name Alternative

GAL, galanin prepropeptide, GALN, GLNN, GMAP, MGC40167, galanin, galanin-message-associated peptide, galanin-related peptide

UniProt

P22466

Reactivity

Human, Mouse, Rat

Immunogen

The immunogen for this antibody is: C-HRSFSDKNGLTSK

Target

GAL

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Neuroscience

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 17kDa band observed in Human Brain (Cerebellum) and in Mouse and Rat Brain lysates (calculated MW of 13.3kDa according to Human NP_057057.2 and of 13.5kDa according to Mouse NP_034383.1 and Rat NP_150240.1) . Recommended concentration: 1-3μg/mL. Primary incubation was 1 hour.Immunohistochemistry:Frozen section of Human Hypothalamus shows staining of galaninergic axons in the human periventricular nucleus. Recommended concentration: 0.2-0.5μg/mL.

Prediction Reactivity

Bovine

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_057057.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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