NRGN Antibody
NRGN Antibody
Product Specifications
Product Name Alternative
NRGN, neurogranin (protein kinase C substrate, RC3), RC3, hng, calmodulin-binding protein, neurogranin, protein kinase C substrate
UniProt
Q92686
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-KIKSGERGRKG
Target
NRGN
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 16kDa band observed in Human Brain (Frontal Cortex) lysate (calculated MW of 7.6kDa according to NP_006167.1) . The observed molecular weights correspond to earlier findings in literature with different antibodies (Li et al, J Biol Chem. 1999 Jan 15;274 (3) :1294-300; PMID: 9880498) . Recommended concentration: 0.1-0.3μg/mL. Preliminary testing was unsuccessful on Mouse Brain for this particular batch.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) . Recommended concentration: 5μg/mL.
Prediction Reactivity
Bovine, Canine, Mouse
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_006167.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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