NUMB Antibody
NUMB Antibody
Product Specifications
Product Name Alternative
NUMB, numb homolog (Drosophila), S171, numb homolog
UniProt
P49757
Reactivity
Human, Mouse, Rat
Immunogen
The immunogen for this antibody is: C-PFSSDLQKTFEIEL
Target
NUMB
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 75kDa band observed in Mouse Brain lysates and in preliminary testing of Mouse Testes lysate (calculated MW of 70.8kDa according to NP_001129547.1) . Recommended concentration: 1-2μg/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cell junctions of A431 and in the cytoplasm of NIH3T3 cells. Recommended concentration: 10μg/mL.
Prediction Reactivity
Bovine, Canine
Tested Applications
ELISA, IF, WB
NCBI Accession Number
001005744.1, NP_001005745.1, NP_003735, NP_001005743.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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