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NUMB Antibody

NUMB Antibody

Product Specifications

Product Name Alternative

NUMB, numb homolog (Drosophila), S171, numb homolog

UniProt

P49757

Reactivity

Human, Mouse, Rat

Immunogen

The immunogen for this antibody is: C-PFSSDLQKTFEIEL

Target

NUMB

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Neuroscience

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 75kDa band observed in Mouse Brain lysates and in preliminary testing of Mouse Testes lysate (calculated MW of 70.8kDa according to NP_001129547.1) . Recommended concentration: 1-2μg/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cell junctions of A431 and in the cytoplasm of NIH3T3 cells. Recommended concentration: 10μg/mL.

Prediction Reactivity

Bovine, Canine

Tested Applications

ELISA, IF, WB

NCBI Accession Number

001005744.1, NP_001005745.1, NP_003735, NP_001005743.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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