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IGFALS Antibody

IGFALS Antibody

Product Specifications

Product Name Alternative

IGFALS, insulin-like growth factor binding protein, acid labile subunit, ALS, INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN COMPLEX ACID LABILE CHAIN

UniProt

P35858

Immunogen

The immunogen for this antibody is: C-GLDLRDLSEAH

Target

IGFALS

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 45kDa band in lysates of cell line HepG2 after 2μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 66.0kDa according to NP_004961.1. The 45kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Prediction Reactivity

Bovine, Canine, Human, Mouse, Porcine, Rat

Tested Applications

ELISA

NCBI Accession Number

NP_004961.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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