ZIC2 Antibody
ZIC2 Antibody
Product Specifications
Product Name Alternative
ZIC2, Zic family member 2 (odd-paired homolog, Drosophila), HPE5, Zic family member 2 (odd-paired Drosophila homolog), Zinc finger protein of the cerebellum 2, zinc finger protein ZIC 2
UniProt
O95409
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-HSGLSSNFNEWY
Target
ZIC2
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:In transfected RWPE1 transiently expressing Human ZIC2-Myc a band of approx. 65kDa is observed. This band is not observed in the non-transfected RWPE1, nor when GFP is transiently expressed. The calculated molecular size is 55.0kDa according to NP_009060.2 and 58.6kDa for the ZIC2-Myc protein. Data provided by Nathan Bowen and Maryam Boseman , Clark Atlanta University, GA, USA.. We have observed an approx 75kDa band in Human Brain (Cerebellum and Cerebral Cortex) lysates Recommended concentration, 0.5-2μg/mL.
Prediction Reactivity
Bovine, Canine, Mouse, Rat
Tested Applications
ELISA, WB
NCBI Accession Number
NP_009060.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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