C22orf28 Antibody
C22orf28 Antibody
Product Specifications
Product Name Alternative
C22orf28, chromosome 22 open reading frame 28, DJ149A16.6, RP1-149A16.6, hypothetical protein LOC51493, novel protein HSPC117
UniProt
Q9Y3I0
Reactivity
Human
Immunogen
The immunogen for this antibody is: C-QADPNKVSARAKKR
Target
C22orf28
Clonality
Polyclonal
Conjugation
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 55kDa band observed in lysates of cell lines HeLa, Jurkat and K562 (calculated MW of 55.2kDa according to NP_055121.1) . Recommended concentration: 0.1-0.3μg/mL.Immunohistochemistry:In paraffin embedded Human Testis shows nuclear and vicinity staining in spermatogonia. Recommended concentration, 5-10μg/mL.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Prediction Reactivity
Bovine, Canine, Mouse, Porcine, Rat
Tested Applications
ELISA, IHC, WB
NCBI Accession Number
NP_055121.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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