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IDH3A Antibody

IDH3A Antibody

Product Specifications

Product Name Alternative

IDH3A, isocitrate dehydrogenase 3 (NAD+) alpha, H-IDH alpha, NAD (+) -specific ICDH subunit alpha, NAD (H) -specific isocitrate dehydrogenase alpha subunit, NAD+-specific ICDH, isocitrate dehydrogenase (NAD+) alpha chain, isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial, isocitric dehydrogenase subunit alpha

UniProt

P50213

Reactivity

Human, Mouse, Porcine, Rat

Immunogen

The immunogen for this antibody is: DFTEEICRRVKDLD

Target

IDH3A

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Metabolism Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 37kDa band observed in lysates of Human Lymph Nodes, and of cell line DAUDI, and 38kDa in Human, Mouse and Rat Spleen lysates and in Pig Spleen and Skeletal Muscle lysates (calculated MW of 39.6kDa according to NP_005521.1) . Recommended concentration: 0.1-0.3μg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Thymus. Recommended concentration: 3.75μg/mL.

Prediction Reactivity

Bovine, Canine

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_005521.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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