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KEAP1 Antibody

KEAP1 Antibody

Product Specifications

Product Name Alternative

KEAP1, kelch-like ECH-associated protein 1, INrf2, KIAA0132, KLHL19, MGC10630, MGC1114, MGC20887, MGC4407, MGC9454, cytosolic inhibitor of Nrf2, kelch-like protein 19

UniProt

Q14145

Reactivity

Human, Mouse

Immunogen

The immunogen for this antibody is: C-EVTPSQHGNRTFS

Target

KEAP1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 75kDa band observed in lysates of cell lines HeLa and NIH3T3 (calculated MW of 69.7kDa according to Human NP_036421.2 and of 69.6kDa according to Mouse NP_057888.1) . Recommended concentration: 0.2-0.6μg/mL.Immunohistochemistry:In paraffin embedded Human Cerebellum shows textured cytoplasm staining in Purkinje cell and processes. Recommended concentration, 3-5μg/mL.Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of HeLa cells. Recommended concentration: 5μg/mL.

Prediction Reactivity

Bovine, Canine, Porcine, Rat

Tested Applications

ELISA, IF, IHC, WB

NCBI Accession Number

NP_036421.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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