MTHFD1L Antibody

Product Specifications

Product Name Alternative

MTHFD1L, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like, DKFZp586G1517, FLJ21145, FTHFSDC1, MTC1THFS, dJ292B18.2, 10-formyl-THF synthetase, formyltetrahydrofolate synthetase domain containing 1, mitochondrial C1-tetrahydrofolate synthase, mitochondrial C1-tetrahydrofolate synthetase, monofunctional C1-tetrahydrofolate synthase, mitochondrial

UniProt

Q6UB35

Reactivity

Human, Mouse, Rat

Immunogen

The immunogen for this antibody is: C-DTNDRFLRKITIGQ

Target

MTHFD1L

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Metabolism Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 110kDa band observed in Human Brain (Cerebellum) (calculated MW of 106kDa according to NP_056255.2) . The same band is observed in lysates of cell line NIH3T3, Mouse Brain and Rat Brain. Recommended concentration: 0.1-0.3μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Pig for this particular batch.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5μg/mL.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Prediction Reactivity

Bovine, Canine, Porcine

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_056255.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.