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NQO1 Antibody

NQO1 Antibody

Product Specifications

Product Name Alternative

NQO1, NAD (P) H dehydrogenase, quinone 1, DHQU, DIA4, DTD, NMOR1, NMORI, QR1, DT-diaphorase, NAD (P) H dehydrogenase [quinone] 1, NAD (P) H:Quinone acceptor oxidoreductase type 1, NAD (P) H:menadione oxidoreductase 1, NAD (P) H:quinone oxidoreductase 1, NAD (P) H:quinone oxireductase, azoreductase, diaphorase (NADH/NADPH) (cytochrome b-5 reductase), diaphorase-4, dioxin-inducible 1, menadione reductase, phylloquinone reductase, quinone reductase 1

UniProt

P15559

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-DKGPFRSKKAVLS

Target

NQO1

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Metabolism Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 30kDa band observed in Human Kidney lysates (calculated MW of 30.9kDa according to NP_000894.1) . Recommended concentration: 0.3-1μg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 3.75μg/mL.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 5μg/mL.

Prediction Reactivity

Bovine, Canine, Porcine

Tested Applications

ELISA, IF, IHC, WB

NCBI Accession Number

NP_000894.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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