PAX4 Antibody
PAX4 Antibody
Product Specifications
Product Name Alternative
PAX4, paired box 4, KPD, MGC129960, MODY9, paired box gene 4, paired domain gene 4
UniProt
O43316
Reactivity
Human, Mouse
Immunogen
The immunogen for this antibody is: C-GKLATATSLPEDTVR
Target
PAX4
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 37kDa band observed in Mouse Pancreas lysates and in lysates of cell lines A431, HeLa and K562 (calculated MW of 37.1kDa according to Human NP_006184.2 and 38.0kDa according to Mouse NP_035168.1) . Recommended concentration: 0.2-0.6μg/mL.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant PAX4 captured by a rabbit antibody. Recommended reporter concentration: 1-2μg/mL
Prediction Reactivity
Canine, Rat
Tested Applications
EIA, ELISA, WB
NCBI Accession Number
NP_006184.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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