MSLN Antibody
MSLN Antibody
Product Specifications
Product Name Alternative
MSLN, mesothelin, CAK1, MPF, SMR, megakaryocyte potentiating factor
UniProt
Q13421
Reactivity
Human
Immunogen
The immunogen for this antibody is: CPSGKKAREIDES
Target
MSLN
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 70kDa band observed in Human Placenta lysates (calculated MW of 68.1kDa according to NP_005814.2 and of 69.0kDa according to NP_037536.2) . Recommended concentration: 0.3-1μg/mL. An additional band of unknown identity was also consistently observed at 150kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Tested Applications
ELISA, WB
NCBI Accession Number
NP_037536.2, NP_005814.2
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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