PON2 Antibody

Product Specifications

Product Name Alternative

PON2, paraoxonase 2, A-esterase 2, aromatic esterase 2, paraoxonase nirs, serum aryldialkylphosphatase 2, serum paraoxonase/arylesterase 2

UniProt

Q15165

Reactivity

Human

Immunogen

The immunogen for this antibody is: C-EKPRARELRIS

Target

PON2

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Neuroscience, Signal Transduction

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 40kDa double band observed in lysates of HepG2 (calculated MW of 39.4kDa according to NP_000296.2 and of 38.0kDa according to NP_001018171.1) . Recommended concentration: 0.2-0.6μg/mL. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Placenta. Recommended concentration: 3.75μg/mL.

Prediction Reactivity

Canine, Porcine, Rat

Tested Applications

ELISA, IHC, WB

NCBI Accession Number

NP_001018171.1, NP_000296.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.