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Oxt Antibody

Oxt Antibody

Product Specifications

Product Name Alternative

Oxt, oxytocin, OT, Oxy, OTTMUSP00000016535

UniProt

P35454

Reactivity

Mouse

Immunogen

The immunogen for this antibody is: DGCRTDPACDPES

Target

Oxt

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Neuroscience

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 22kDa band in Mouse Liver after 1μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for this band, given the calculated size of 12.9kDa according to NP_035155.1. The 22kDa band was successfully blocked by incubation with the immunizing peptide.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes of NIH3T3 cells. Recommended concentration: 10μg/mL. Flow Cytometry: Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 10μg/mL.

Prediction Reactivity

Rat

Tested Applications

ELISA, FC, IF

NCBI Accession Number

NP_035155.1

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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