TRIM71 Antibody
TRIM71 Antibody
Product Specifications
Product Name Alternative
TRIM71, tripartite motif-containing 71, LIN-41, LIN41, abnormal cell LINeage LIN-41, homolog of C. elegans Lin-41
UniProt
Q2Q1W2
Reactivity
Mouse
Immunogen
The immunogen for this antibody is: C-NKLESTISAVQ
Target
TRIM71
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 100kDa band observed in lysates of cell line NIH3T3 (calculated MW of 93.4kDa according to Human NP_001034200.1 and of 92.1kDa according to Mouse NP_001035968.1) . Recommended concentration: 1-3μg/mL. An additional band of unknown identity was also consistently observed at 14kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Prediction Reactivity
Bovine, Canine, Human, Rat
Tested Applications
ELISA, WB
NCBI Accession Number
NP_001034200.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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