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POU3F2 Antibody

POU3F2 Antibody

Product Specifications

Product Name Alternative

POU3F2, POU class 3 homeobox 2, BRN2, OCT7, OTF7, POUF3, POU domain, class 3, transcription factor 2

UniProt

P20265

Reactivity

Human, Mouse, Rat

Immunogen

The immunogen for this antibody is: C-AQSLVQGDYGALQSN

Target

POU3F2

Clonality

Polyclonal

Conjugation

Unconjugated

Field of Research

Neuroscience

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 ug/mL

Form

Liquid

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 45kDa band observed in Human Brain (Cerebellum) and in Mouse and Rat Brain lysates (calculated MW of 46.9kDa according to Human NP_005595.2 and 47.1kDa according to Mouse NP_032925.1) . Recommended concentration: 0.1-0.3μg/mL.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Prediction Reactivity

Bovine, Porcine

Tested Applications

ELISA, WB

NCBI Accession Number

NP_005595.2

Host or Source

Goat

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

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