POU5F1B Antibody
POU5F1B Antibody
Product Specifications
Product Name Alternative
POU5F1B, POU class 5 homeobox 1B, OCT4-pg1, OTF3C, OTF3P1, POU5F1P1, POU5FLC20, POU5FLC8, POU 5 domain protein, POU class 5 homeobox 1 pseudogene 1, POU domain transcription factor Oct-4, POU domain, class 5, transcription factor 1 pseudogene 1, octamer binding protein 3_like sequence
UniProt
Q06416
Immunogen
The immunogen for this antibody is: C-TPGYGSPHFTALYS
Target
POU5F1B
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Epigenetics & Chromatin
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 ug/mL
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 28kDa band in Human Heart lysates after 1μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 38.6kDa according to NP_001153014.1. The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Prediction Reactivity
Human
Tested Applications
ELISA
NCBI Accession Number
NP_001153014.1
Host or Source
Goat
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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